Auto & Motor

Learning The “Secrets” of Sequencing

The Process Involved in the Extraction and Purification of DNA.

Deoxyribonucleic Acid, popularly known as DNA is an element found in any living thing. This molecule is known to contain a genetic code for every organism hence it is a blueprint that continuously acts in plans of any substance that is produced by these organisms at the course of their living. Interestingly, as popular as the term DNA appears to be, very few people have had a chance to see the molecule simply because the molecule happens to be very small for one to easily see. However, one can simply do extraction on the molecules easily at home or in the school using available household materials like plants and especially peas.

Some of the essential materials you will need for the extraction process include alcohol, meat tenderizer, split peas, dishwashing detergent probably in liquid form, a blender, a beaker or clear glass container, a stirring rod or a spoon. To begin with, blend at least a half cup of split peas in a cupful of water. Having obtained a soupy mixture from the blend, use a homogenous solution to separate the mixture. One may also consider reducing the mixture and spare some just in case. Then, pour some of the solutions you have, an adequate amount of it into a container up to a level of at least two inches.

In the third step, you need to add at least a tablespoonful of the liquid dishwashing detergent on top of the solution. Then stir the mixture slowly for about five minutes. In the next procedure; add a pinch of the meat tenderizer on the mixture then stir for another five minutes. After stirring the mixture, drizzle some alcohol above the container. Consider adding the alcohol until you obtain a layer of three inches on the split peas solution. Peas are mostly used for this extraction because they are so rich in the molecule. Again the detergent is used to break down the membrane cell for it to release its contents and the meat tenderizer to dissolve the membrane nuclear for it to release the DNA inside. Use of the alcohol in the process is to bring out the DNA strands.

During the purification process, there are a variety of processing kits that can be used. This simply is attributed by the fact that different samples work with different kind of tissues, forensic samples, plant parts and also different cellular structures. Continuously, there are new inventions being made on machines and cutting edge products through different forms of amplification and facilitations for use in new projects and experiments. One such way involves use of magnetized beads which happens to be very effective. Another very helpful way of the molecule purification involves, use of plasmid purification kits which produces low endotoxins levels for use in the subject transfection.

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